Shahid Sadoughi University of Medical Sciences https://en.ssu.ac.ir/publications/ Advances in Pharmacology and Therapeutics Journal 2783-3445 1 1 2021 09 01 Healing effects of Solanum nigrum fruit extract on second-degree burn wounds and its antibacterial activity against common pathogens of burn infection 1 12 186 https://doi.org/10.18502/aptj.v1i1.7915 EN Parisa Rashidi Ashjerdi Mohsen Zabihi Ali Mohammad Ranjbar Seyed Hossein Hekmatimoghaddam Mehdi Fatahi Bafghie Journal Article 2021 10 08 <strong>Aims:</strong> <em>Solanum nigrum</em> (<em>S. nigrum</em>) is a species of flowering plant from <em>the Solanaceae</em> family and one of the indigenous plants of Eurasia. Given the biological activities of this plant, like antimicrobial, antioxidant, and anti-inflammatory ones, this study assessed its effects on the healing process of second-degree burn wounds in rats. We also evaluated its antibacterial activity against common pathogens of burn wound infection (i.e., <em>Pseudomonas aeruginosa</em>, <em>Staphylococcus aureus,</em> and <em>Acinetobacter baumanni</em>).<br /><strong>Methods:</strong> <em>S. nigrum</em> fruit extract was prepared by percolation and reflux methods. The extract was applied for the treatment of animal models with second-degree burn wounds. Parameters of wound healing and maturation, including collagen deposition, epithelialization, reduction of neutrophil migration, and angiogenesis, were evaluated. The antimicrobial activity of <em>S. nigrum</em> fruit extract against common pathogens of burn wound infection was assessed by the agar well diffusion method via measurement of zones of microbial growth inhibition.<br /><strong>Results:</strong> Histological analysis showed a significant reduction in neutrophil migration by the 20% hydroalcoholic extract vs. control group (normal saline). In addition, we found that the 20% hydroalcoholic extract was more efficient than silver sulfadiazine in augmenting collagen deposition. <em>S. nigrum</em> hydro alcoholic extract also showed an inhibitory effect on <em>S. aureus</em>.<br /><strong>Conclusion: </strong><em>S. nigrum</em> 20% hydroalcoholic extract improved some of the wound healing parameters such as collagen deposition and inflammation. It also shows an inhibitory effect on <em>S. aureus</em>. So, it may have therapeutic effects on burns. https://aptjournal.ssu.ac.ir/article_186_bd12df2646e056c204c682d77a316810.pdf